This paper reports a single-pulse analysis of five pulsars using the Five-hundred-meter Aperture Spherical radio Telescope (FAST). The analysis results show that pulse nulling is detected in all five pulsars. Among them, the pulse nulling of PSR J1907+4002 is reported for the first time. The nulling fractions (NFs) are calculated. The values for PSRs J0304+1932, J0528+2200, J0754+3231, J1115+5030 and J1907+4002 are 14.50% ± 1.00%, 28.50% ± 2.00%, 32.10% ± 1.50%, 60.40% ± 1.50% and 12.20% ± 0.90%, respectively. The newly measured NFs are compared with those from published literature. No firm evidence was found for intrinsic frequency dependence of the NFs in these pulsars. A check for correlations among NFs, spin period, and characteristic ages shows no obvious dependence. It should be noted that these correlations require further investigation using observations from a larger pulsar sample, as the current sample size is too limited to confirm any definitive correlation. Furthermore, a discrete Fourier transform analysis of pulse nulling periodicity reveals that these pulsars may exhibit quasi-periodic nulling. An analysis of two-dimensional fluctuation spectra further indicates that the periodic amplitude modulation observed in these pulsars may arise from pulse nulling. Subpulse drifting is only observed in PSRs J0528+2200 and J1115+5030. This work not only enriches the observational sample of pulse nulling and subpulse drifting, but also provides important observational constraints for further understanding the radiation mechanisms in pulsar magnetospheres.