This study addresses the need for real-time sampling and transmission of wideband signals in radio astronomy, developing a comprehensive technical workflow from hardware implementation to firmware compilation. The system, based on the Xilinx ZCU111 Radio Frequency System on Chip (RFSoC) development board, incorporates hardware modifications and custom development using the CASPER open-source toolflow. We adopt a channelization method based on the Polyphase Filter Bank algorithm, provide the corresponding theoretical derivations, and implement the scheme on the CASPER open-source toolflow. The developed firmware enables channelization (16 channels, 128 MHz bandwidth) of ultra-wideband signals at a 4096 MHz sampling rate on RFSoC hardware. Deployed at the Nanshan 26 m radio telescope for L-band (964–1732 MHz) observations, the system successfully produced high signal-to-noise ratio pulsar profiles after processing with DSPSR software, validating the reliability of the channelization algorithm. These results confirm the feasibility of RFSoC-based architectures for wideband signal channelization, offering a practical pathway for future astronomical backends.